683 MycoKeys
MycoKeys 108: 197-225 (2024) DOI: 10.3897/mycokeys.108.126712
Research Article
Stromatolinea, a new diatrypaceous fungal genus (Ascomycota, Sordariomycetes, Xylariales, Diatrypaceae) from China
Kamran Habib'23®, Xin Zhou?, Wenyu Zeng?, Xu Zhang2, Hongmin Hu2®, Qianzhen Wu®, Lili Liu’, Yan Lin’, Xiangchun Shen", Jichuan Kang™, Qirui Li??©
1 State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, 550025, China
2 The High Efficacy Application of Natural Medicinal Resources Engineering Centre of Guizhou Province (The Key Laboratory of Optimal Utilization of Natural Medicine Resources), School of Pharmaceutical Sciences, Guizhou Medical University, Gui‘an New District, 561113, China Department of Botany, Khushal Khan Khattak University, Karak, KP Pakistan
4 Immune Cells and Antibody Engineering Research Centre of Guizhou Province/Key Laboratory of Biology and Medical Engineering, Guizhou Medical University,
Gui'an New District, 561113, China
5 Engineering and Research Centre for Southwest Bio-Pharmaceutical, Resources of National Education Ministry of China, Guizhou University, Guiyang, China Corresponding author: Qirui Li (Iqrnd2008@163.com)
OPEN Qaceess
Academic editor: Thorsten Lumbsch Received: 2 May 2024
Accepted: 2 August 2024
Published: 4 September 2024
Citation: Habib K, Zhou X, Zeng W, Zhang X, Hu H, Wu Q, Liu L, Lin Y, Shen X, Kang J, Li Q (2024) Stromatolinea,
a new diatrypaceous fungal genus (Ascomycota, Sordariomycetes, Xylariales, Diatrypaceae) from China. Mycokeys 108: 197-225. https://doi. org/10.3897/mycokeys. 108.126712
Copyright: © Kamran Habib et al.
This is an open access article distributed under terms of the Creative Commons Attribution License (Attribution 4.0 International - CC BY 4.0).
Abstract
Molecular phylogeny and morphological characteristics of collections of diatrypaceous fungi from Guizhou Province, China, lead to the establishment of a new genus, Stromat- olinea, and the identification of four new species and two new combinations. The taxa were found growing on the dead culms of Phyllostachys bamboo. The new genus is distinguished by its well-developed, discrete linear stromata with yellow interior tissue and allantoid subhyaline ascospores. The newly described species are Stromatolinea grisea, S. guizhouensis, S. hydei, and S. xishuiensis. Additionally, two new combinations, Stromatolinea linearis and S. phaselina, are proposed based on comparative analysis and morphology. Phylogenetic analyses were conducted using ITS and TUB2 sequenc- es. The study includes comprehensive morphological descriptions, illustrations, and a phylogenetic tree depicting the placement of the new taxa.
Key words: 1 new genus, 4 new species, bambusicolous fungi, fungal systematics, Guizhou Province
Introduction
In recent years, several new genera within Diatrypaceae have been reported through a combination of morphological characteristics and multi-locus phy- logeny. Currently, the family is represented by 27 genera, i.e., Allocryptovalsa, Allodiatrype, Alloeutypa, Anthostoma, Cryptosphaeria, Cryptovalsa, Diatrypasi- milis, Diatrype, Diatrypella, Dothideovalsa, Echinomyces, Endoxylina, Eutypa, Eu- typella, Halocryptosphaeria, Halocryptovalsa, Halodiatrype, Leptoperidia, Liber- tella, Monosporascus, Neoeutypella, Paraeutypella, Pedumispora, Peroneutypa, Pseudodiatrype, Quaternaria, and Rostronitschkia (Hyde et al. 2020; Konta et al. 2020; Dissanayake et al. 2021; Long et al. 2021; Samarakoon et al. 2022; Wijayawardene et al. 2022; Chen et al. 2023; Ma et al. 2023).
197
Kamran Habib et al.: A new Diatrypaceous fungal genus
Diatrypaceae species are distributed worldwide and are commonly found on deadwood and the bark of various plant species. The family is characterized by black or dark brown, immersed to erumpent, pseudostromatic or eustromatic stromata, polysporous or 8-spored asci, hyaline to light brown allantoid asco- spores, and a libertella-like asexual morph (Senanayake et al. 2015; Wijayawar- dene et al. 2017).
Bamboo, as the largest member of the grass family Poaceae, plays an im- portant role in local economies worldwide, being distributed across diverse climates, from cold mountainous regions to hot tropical areas. China boasts plentiful bamboo resources, with its bamboo species constituting over 50% of the world’s total (Liu et al. 2018). There are more than 1300 fungal species as- sociated with bamboo, including 150 basidiomycetes, 800 ascomycetes. The taxonomic placements of bamboo-associated ascomycetous fungi are highly diverse, comprising over 1,150 species, in 120 families and 400 genera (Dai et al. 2018; Hyde et al. 2023). Jiang et al. (2022) reported 512 bambusicolous as- comycetous taxa from China, associated with 16 bamboo genera, representing more than one-third of the known bambusicolous ascomycetes in the world.
In an investigation into the diversity of bambusicolous fungi in Guizhou, Chi- na, four previously undescribed species of diatrypaceous fungi were discov- ered. Morphological analyses revealed their close affinity to the genus Alloeu- typa. However, phylogenetic analyses did not support their placement within this genus. Following detailed morphological examinations and comparative analyses, we propose a new genus, Stromatolinea, within the family Diatrypa- ceae, which includes four new species and one new combination. The findings contribute significantly to the understanding of diatrypaceous fungal diversity and taxonomy.
Materials and methods Sample collection and morphological study
The specimens of bamboo were collected during surveys conducted in the Guizhou province, China. All related habitat information, including details about elevation, climatic conditions, and geographical features, was recorded. The photos of the collected materials were taken using a Canon G15 camera (Can- on Corporation, Tokyo, Japan). Materials were placed in paper bags and were taken to the lab for examination. To preserve the freshness of the specimens, they were dried at room temperature. The dried specimens were carefully la- beled and stored in an ultra-low freezer at —80 °C for one week to eliminate any insects and their eggs. After this preparation, the specimens were ready for both morphological and molecular studies.
Macroscopic characteristics were observed under an Olympus SZ61 stereo- microscope and photographed with a Canon 70OD digital camera fitted to a light microscope (Nikon Ni). Morphological characteristics of specimens were examined, and photomicrographs were taken as described in Senanayake et al. (2020). Materials were mounted in water for anatomical examination and add- ed Melzer’s reagent where necessary. More than 30 ascospores and 30 asci were measured using the Tarosoft image framework (v. 0.9.0.7). Images were arranged using Adobe Photoshop CS6 (Adobe Systems, USA).
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 198
Kamran Habib et al.: Anew Diatrypaceous fungal genus
Isolates were derived by single spore isolation following the method of Chomnunti et al. (2014). Germinating spores were observed with a Stereo Zoom microscope and transferred to potato dextrose agar (PDA; 39 g/I distilled water, Difco potato dextrose). The cultures were incubated at 25-30 °C for 1-4 weeks, with frequent observations. Cultural characteristics, such as mycelium colour, shape, texture and growth rate, were recorded after incubating at 25 °C under normal light for a week.
Herbarium materials were deposited at the herbarium of Guizhou Medical University (GMB) and the Herbarium of Cryptogams, Herbarium of Kunming In- stitute of Botany, Chinese Academy of Sciences (KUN-HKAS), and living cultures were deposited at the Guizhou Medical University Culture Collection (GMBC).
DNA extraction, PCR amplification, and sequencing
Fungal DNA was directly extracted from the contents of stromata and perithe- cia using the BIOMIGA fungus genomic DNA extraction kit, following the man- ufacturer’s instructions. The DNA samples were stored at —20 °C. Internal tran- scribed spacers (ITS), and B-tubulin (TUB2), were amplified by PCR with primers ITS1/ITS4 (White et al. 1990; Gardes and Bruns 1993), and T1/T22 (Glass and Donaldson 1995; O’Donnell and Cigelnik 1997), respectively. The components of a 25 uL volume PCR mixture was: 9.5 uL of double distilled water, 12.5 uL of PCR Master Mix, 1 uL of each primer and 1 uL of template DNA. Qualified PCR products were checked through 1.5% agarose gel electrophoresis stained with GoldenView, and sent to Sangon Co., China, for sequencing (Xie et al. 2020).
Phylogenetic analyses
All newly generated sequences from this study were deposited in GenBank (https:// www.ncbi.nim.nih.gov/; accessed on March 28, 2024; Table 1). These sequences were compared with each other and all the known sequences in the GenBank by using the BLAST algorithm for identification. The sequences retrieved from open databases originated from Li et al. (2023), Ma et al. (2023), and the BLASTn results of close matches and other Diatrypaceae representatives. The molecular phyloge- ny was inferred from a combined dataset of ITS and TUB2 sequences. Sequences were aligned using the MAFFT v.7.110 online programme (Katoh et al. 2019) with the default settings, respectively. Alignment was adjusted manually using BioEdit v.7.0.5.3 (Hall 1999) where necessary. The start and end of alignment were trimmed to nearly equal number of sites for all sequences. The combined sequence data was used to perform maximum likelihood (ML) and Bayesian inference analysis (BI). The ML analysis was implemented in RAXxML v.8.2.12 using the GTRGAMMA substitution model with 1,000 bootstrap replicates (Stamatakis 2014).
The Bayesian inference analysis was performed in MrBayes v. 3.2.1 (Ron- quist et al. 2012). The model of evolution was estimated by MrModeltest 2.2 (Nylander 2004). The Markov chain Monte Carlo (MCMC) sampling in MrBayes v.3.2.2 (Ronquist et al. 2012) was used to determine the posterior probabilities (PP). Six simultaneous Markov chains were run for 1,000,000 generations, and trees were sampled every 1000" generation. The phylogenetic tree was visual- ized in FIGTREE v.1.4.3 (Rambaut 2012). All analyses were run on the CIPRES Science Gateway v 3.3 web portal (Miller et al. 2010).
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 199
Kamran Habib et al.: Anew Diatrypaceous fungal genus
Table 1. GenBank Accession Numbers used in this study. The newly generated sequences are marked bold. T indicates
type strain.
Species Allocryptovalsa castaneae Allocryptovalsa castaneicola Allocryptovalsa cryptovalsoidea Allocryptovalsa elaeidis Allocryptovalsa elevata Allocryptovalsa polyspora Allocryptovalsa rabenhorstii Allocryptovalsa sichuanensis Allocryptovalsa xishuangbanica Allocryptovalsa xishuangbanica Allodiatrype albelloscutata Allodiatrype arengae Allodiatrype elaeidicola Allodiatrype elaeidis Allodiatrype taiyangheensis Allodiatrype thailandica Allodiatrype trigemina Alloeutypa flavovirens Alloeutypa milinensis Alloeutypa milinensis Anthostoma decipiens Anthostoma decipiens Cryptosphaeria eunomia var. eunomia Cryptosphaeria eunomia var. fraxini Cryptosphaeria ligniota Cryptosphaeria pullmanensis Cryptosphaeria subcutanea Cryptosphaeria subcutanea Cryptovalsa ampelina Cryptovalsa ampelina Cryptovalsa elevata Diatrype betulaceicola Diatrype betulae Diatrype betulae Diatrype bullata Diatrype camelliae-japonicae Diatrype camelliae-japonicae Diatrype castaneicola Diatrype disciformis Diatrype disciformis Diatrype enteroxantha Diatrype enteroxantha Diatrype enteroxantha
Diatrype lancangensis
Isolate/specimen voucher | CFCC52428" CFCC52432'
HVFIGO2 MFLUCC 15-0707" WAO08CB MFLUCC 17-0364" GMB0416 HKAS 1070177 KUMCC 21-0830 GMB0417 IFRD 9100 ° MFLUCC 15-0713" MFLUCC 15-0737a MFLUCC 15-0708a IFRDCC2800' MFLUCC 15-3662" FCATAS 842 E48C FCATAS4309" FCATAS4382 IPV-FW349 JL567 Cie C5C CBS 273.87 ATCC 52655 DSUB100A CBS 240.87" A001 DRO101 CBS 125574 FCATAS 2725' CFCC52416' GMB0426 UCDDCh400 GMB0427' GMB0428 CFCC52425' GNA14 D21C HUEFS155114 HUEFS155116 GMB0433 GMB0045'
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712
Reference Zhu et al. 2021 Zhu et al. 2021 Trouillas et al. 2011 Konta et al. 2020 Trouillas et al. 2011 Senwanna et al. 2017 Li et al. 2023 Samarakoon et al. 2022 Maharachchikumbura et al. 2022 Li et al. 2023 Li et al. 2022 Konta et al. 2020 Konta et al. 2020 Konta et al. 2020 Li et al. 2022 Li et al. 2016 Peng et al. 2021 Rolshausen et al. 2006 Ma et al. 2023 Ma et al. 2023 Unpublished Luque et al. 2012 Acero et al. 2004 Acero et al. 2004 Acero et al. 2004 Trouillas et al. 2015 Trouillas et al. 2015 Trouillas et al. 2015 Trouillas et al. 2010 Trouillas et al. 2010 Vu et al. 2019 Yang et al. 2022 Zhu et al. 2021 Li et al. 2023 Rolshausen et al. 2006 Li et al. 2023 Li et al. 2023 Zhu et al. 2021 Senanayake et al. 2015 Acero et al. 2004 de Almeida et al. 2016 de Almeida et al. 2016 Li et al. 2023 Long et al. 2021
ITS MW632945 MW632947 HQ692573 MN308410 HQ692619 MF959500
OP935171 MW240633 ON041128 OP935176 OK257020 MN308411 MN308415 MN308412 OK257021 NR164240 MW031919 AJ302457 OP538689 OP538690 AM399021 JN975370 AJ302417 AJ302421 KT425233 KT425235 KT425189 KT425232 GQ293901 GQ293902 MH863711 OM040386 MW632943 OP935181 DQ006946 OP933172 OP935173 MW632941 KR605644 AJ302437 KM396617 KM396618 OP935170 MW797113
B-tubulin MW656393 MW656395 HQ692524 MN340296 HQ692523 MG334556 OP938733 MW775592 ON081498 OP938739
NA MN340297 MN340299 MN340298 OK345036
NA MW371289 DQ006959 OP557595 OP557596 AM920693 JN975407
NA
NA KT425168 KT425170 KT425124 KT425167 GQ293972 GQ293982
NA OM240966 MW656391 OP938750 DQ007002 OP938734 OP938735 MW656389 KY352434
NA KT003700 KT022236 OP938736 MW814885
200
Kamran Habib et al.: Anew Diatrypaceous fungal genus
Species Diatrype lancangensis Diatrype larissae Diatrype lijiangensis Diatrype palmicola Diatrype palmicola Diatrype quercicola Diatrype rubi Diatrype rubi Diatrype spilomea Diatrype stigma Diatrype undulata Diatrypella atlantica Diatrypella atlantica Diatrypella banksiae Diatrypella betulae Diatrypella betulicola Diatrypella delonicis Diatrypella delonicis Diatrypella elaeidis Diatrypella fatsiae-japonica Diatrypella fatsiae-japonicae Diatrypella favacea Diatrypella favacea Diatrypella frostii Diatrypella guiyangensis Diatrypella guiyangensis Diatrypella heveae Diatrypella heveae Diatrypella hubeiensis Diatrypella iranensis Diatrypella longiasca Diatrypella macrospora Diatrypella oregonensis (Diatrype oregonensis) Diatrypella oregonensis (Diatrype oregonensis) Diatrypella pseudooregonensis Diatrypella pseudooregonensis Diatrypella pulvinata Diatrypella pulvinata Diatrypella tectonae Diatrypella tectonae Diatrypella verruciformis Diatrypella verruciformis Diatrypella vulgaris Diatrypella vulgaris Diatrypella yunnanensis Eutypa armeniacae
Eutypa astroidea
Isolate/specimen voucher
GMBO0046 FCATAS 2723' MFLU 19-0717° MFLU 15-0040° MFLU 15-0041
CFCC52418°
GMB0429'
GMB0430
D17C DCASH200 D20C HUEFS 136873 LCM 888.01 CPE:29118" CFCC52406' CFCC52411° MFLUCC 15-1014" MFLU 16-1032 MFLUCC 15-0279° GMB0422' GMB0423 380 DL26C UFMGCB 1917 GMB0414' GMB0415 MFLUCC 15-0274 MFLUCC 17-0368° CFCC 52413" KDQ18' KUMCC 20-0021" KDQ15 DPL200 CA117 GMB0039 GMBO0041' H048 DL29C MFLUCC 12-0172a"™ MFLUCC 12-0172b" UCROK1467
UCROK754
HVFRAO2
HVGRFO3
VT01 ATCC 28120 CBS 292.87
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712
Reference Long et al. 2021 Yang et al. 2022 Thiyagaraja et al. 2019 Liu et al. 2015 Liu et al. 2015 Zhu et al. 2021 Li et al. 2023 Li et al. 2023 Acero et al. 2004 Trouillas et al. 2010 Acero et al. 2004 de Almeida et al. 2016 Unpublished Crous et al. 2016 Zhu et al. 2021 Zhu et al. 2021 Hyde et al. 2019 Hyde et al. 2019 Konta et al. 2020 Li et al. 2023 Li et al. 2023 de Almeida et al. 2016 Unpublished Vieira et al. 2011 Li et al. 2023 Li et al. 2023 Konta et al. 2020 Senwanna et al. 2017 Zhu et al. 2021 Mehrabi et al. 2015 Dissanayake et al. 2021 Mehrabi et al. 2016 Trouillas et al. 2010 Trouillas et al. 2010 Long et al. 2021 Long et al. 2021 de Almeida et al. 2016 Unpublished Shang et al. 2017 Shang et al. 2017 Lynch et al. 2013 Lynch et al. 2013 Trouillas et al. 2011 Trouillas et al. 2011 Zhu et al. 2021 Rolshausen et al. 2006 Rolshausen et al. 2006
ITS MW797114 OM040384 MK852582 NR185365
KP744439 MW632938 OP935182 0P935183 AJ302433 GQ293947 AJ302436 KM396614 MF495421 KY173402 MW632931 MW632935 MH812994 MH812995 MN308417 OP935184 OP935185 KU320616 AJ302440 HQ377280 OP935188 OP935189 MN308418 MF959501 MW632937 KM245033 MW036141 KR605648 GQ293940 GQ293934 MW797115 NR174917 FR715523 AJ302443 KY283084 KY283085 JX144793 JX144783 HQ692591 HQ692590 MN653008 DQ006948 AJ302458
B-tubulin MW814886 OM240964 MK852583
NA
NA MW656386 OP938740
OP938741
NA GQ294003
NA KR259647
NA MW656379 MW656383 MH847790 MH847791 MN340300 OP938744 OP938745
NA
NA
NA OP938742 OP938743 MN340301 MG334557 MW656385
KY352429 MW239658 KY352430 GQ293999 GQ293996 MW814888 MW814890 FR715495 NA NA KY421043 JX174093 JX174083 HQ692503 HQ692502 MN887112 DQ006975 DQ006966
201
Kamran Habib et al.: Anew Diatrypaceous fungal genus
Species Eutypa camelliae Eutypa cerasi Eutypa cerasi Eutypa laevata Eutypa lata Eutypa lata Eutypa lata Eutypa lejoplaca Eutypa leptoplaca Eutypa maura Eutypa microasca Eutypa sparsa Eutypa tetragona Eutypella caricae Eutypella cearensis Eutypella cerviculata Eutypella cerviculata Eutypella leprosa Eutypella leprosa Eutypella microtheca Eutypella motuoensis Eutypella motuoensis Eutypella parasitica Eutypella quercina Eutypella semicircularis Eutypella tamaricis Halocryptovalsa salicorniae Halodiatrype avicenniae Halodiatrype salinicola kKretzschmaria deusta Monosporascus cannonballus Monosporascus cannonballus Neoeutypella baoshanensis Paraeutypella citricola Paraeutypella citricola Paraeutypella guizhouensis Paraeutypella pseudoguizhouensis Paraeutypella pseudoguizhouensis Paraeutypella vitis Paraeutypella vitis Pedumispora rhizophorae Pedumispora rhizophorae Peroneutypa alsophila Peroneutypa curvispora Peroneutypa diminutiasca Peroneutypa diminutispora
Peroneutypa hainanensis
Isolate/specimen voucher
HKAS 107022' GMBO0048' GMBO0049
E40C CBS 291.87 CBS290.87 EP18 RGAO1 CBS 248.87 CBS 287.87 CBS 219.87 BAFC 51550 3802 3b CBS 284.87 EL51C HUEFS 131070 M68 EL59C EL54C 60 BCMX01 FCATAS4035 FCATAS4082' CBS 210.397 IRANC2543C" MP4669 MFLUCC 14-0445 MFLUCC 15-0185 MFLUCC 15-0953 MFLUCC 15-1277 CBS 826.72 CMM3646 ATCC:2693' HMAS:255436 HVVITO7 HVGRFO1 KUMCC 20-0017 GMB0420' GMB0421 UCD2291AR UCD2428TX BCC44877 BCC44878 EL58C HUEFS 136877 MFLUCC 17-2144"
HUEFS 192196 GMB0424'
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712
Reference Samarakoon et al. 2022 Long et al. 2021 Long et al. 2021 Acero et al. 2004 Trouillas and Gubler, 2010 Trouillas et al. 2011 Trouillas et al. 2011 Rolshausen et al. 2006 Rolshausen et al. 2006 Rolshausen et al. 2006 Grassi et al. 2014 Trouillas and Gubler, 2004 Rolshausen et al. 2006 Acero et al. 2004 de Almeida et al. 2016 Arhipova et al. 2012 Acero et al. 2004 Acero et al. 2004 de Almeida et al. 2016
Paolinelli-Alfonso et al. 2015
Ma et al. 2023 Ma et al. 2023 Jurc et al. 2006 Mehrabi et al. 2019 Mehrabi et al. 2016 Thambugala et al. 2017 Dayarathne et al. 2020b Dayarathne et al. 2016 Dayarathne et al. 2016 U’ren et al. 2016 Unpublished Unpublished Phookamsak et al. 2019 Trouillas et al. 2011 Trouillas et al. 2011 Dissanayake et al. 2021 Li et al. 2023 Li et al. 2023 Urbez-Torres et al. 2012 Urbez-Torres et al. 2009 Klaysuban et al. 2014 Klaysuban et al. 2014 Acero et al. 2004 de Almeida et al. 2016 Shang et al. 2018 de Almeida et al. 2016 Li et al. 2023
ITS MW240634 MW797104 MW797105
AJ302449 HM164736 HQ692611 HQ692614 DQ006922 DQ006924 DQ006926 KF964566 AY684220 DQ006923 AJ302460 KM396639 JF340269 AJ302468 AJ302463 KU320622 KC405563 OP538695 OP538693 DQ118966 KX828139 JQ517314 KU900330 MH304410 KX573916 KX573915 KU683767 JX971617 FJ430598 NR164038 HQ692579 HQ692589 MW036141 OP935186 OP935187 HQ288224 FJ790851 KJ888853 KJ888854 AJ302467 KM396641 MG873479 KM396647 OP9SSI179
B-tubulin MW775593 MW814893 MW814877
NA HM164770 HQ692501 HQ692497 DQ006974 DQ006961 DQ006967
KF964572 AY684201 DQ006960
NA
NA
NA
NA
NA
NA KC405560
NA OP557599
NA KY352449
NA KX453302 MH370274 KX573931 KX573932 KU684190
NA
NA MH822888 HQ692512 HQ692521 MW239661 OP938748 OP938749 HQ288303 GU294726
NA
NA
NA
NA MH316765
NA OP938746
202
Kamran Habib et al.: Anew Diatrypaceous fungal genus
Species Peroneutypa hainanensis Peroneutypa indica Peroneutypa kochiana Peroneutypa kunmingensis Peroneutypa leucaenae Peroneutypa longiasca Peroneutypa mackenziei Peroneutypa mangrovei Peroneutypa polysporae Peroneutypa gianensis Peroneutypa gianensis Peroneutypa rubiformis Pseudodiatrype hainanensis Pseudodiatrype hainanensis Quaternaria quaternata Quaternaria quaternata Stromatolinea grisea Stromatolinea grisea Stromatolinea guizhouensis Stromatolinea guizhouensis Stromatolinea hydei Stromatolinea hydei Stromatolinea hydei Stromatolinea linearis Stromatolinea linearis Stromatolinea xishuiensis Stromatolinea xishuiensis Stromatolinea xishuiensis Vasilyeva cinnamomi Vasilyeva cinnamomi
Xylaria hypoxylon
Isolate/specimen voucher GMB0425 NFCCI 43937 EL53M HKAS 113189° MFLU 18-0816" MFLU 17-1217 MFLUCC 16-0072" PUFD526 NFCCI 4392" GMB0431' GMB0432 MFLUCC 17-2142" GMB0054" GMB0055 EL60C GNF13 GMB4512 GMB4508 GMB4523 GMB4515 GMB4509 GMB4538 GMB4521 MFLUCC 11-0503 MFLUCC 15-0198 GMB4535 GMB4522 GMB4514 GMB0418' GMB0419 CBS 122620
Results
Phylogenetic analyses
Reference Li et al. 2023 Dayarathne et al. 2020a Carmaran et al. 2006 Phukhamsakda et al. 2022 Samarakoon et al. 2022 Senwanna et al. 2017 Shang et al. 2017 Phookamsak et al. 2019 Dayarathne et al. 2020a Li et al. 2023 Li et al. 2023 Shang et al. 2018 Long et al. 2021 Long et al. 2021 Acero et al. 2004 Mehrabi et al. 2016 This study This study This study This study This study This study This study Dai et al. 2017 Dai et al. 2017 This study This study This study Li et al. 2023 Li et al. 2023 Persoh et al. 2009
ITS OP935180 MN061368 AJ302462 MZ475070 MW240631 MF959502 KY283083 MG844286 MN061367 OP935177 OP935178 MG873477 MW797111 MW797112 AJ302469 KR605645 PQ113920 PQ113921 PQ113922 PQ113923 PQ113924 PQ113925 PQ113926 KU940150 KU940149 PQ113927 PQ113928 PQ113929 OP935174 OP935175 AM993141
B-tubulin OP938747 MN431498
NA MZ490589 MW775591 MG334558
KY706363 MH094409 MN431497
NA
NA MH316763 MW814883 MW814884
NA
NA PQ115208 PQ115209 PQ115210 PQ115211 PQ115212 PQ115213 PQ115214 MW775587 PQ115215 PQ115216 PQ115217 OP938737 OP938738
KX271279
After the exclusion of ambiguously aligned regions and long gaps, the final combined data matrix contained 1,450 characters. Kretzschmaria deusta (CBS 826.72) and Xylaria hypoxylon (CBS 122620) were added as the outgroup. The tree topology derived from Maximum Likelihood (ML) analysis closely resem- bled that of Bayesian Inference (BI) analysis. The best-scoring RAXxML tree is shown in Fig. 1. The topology of the phylogenetic tree is similar to those in previous studies (Li et al. 2023; Ma et al. 2023). The new genus Stromatolinea, including four species, formed a distinct clade that represents its monophyletic status. The strains ZHKUCC 21-0114 and S21 clustered in the clade of the new genus, are deposited in NCBI under the name Diatrypella sp., but remain unpub- lished. The details provided in NCBI for these two strains (ZHKUCC 21-0114 and S21) were searched, but no records were found.
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712
203
Kamran Habib et al.: A new Diatrypaceous fungal genus
A Diatrypella delonicis MFLUCC 15-1014
ad Diatrypella delonicis RHP 77 Diatrypella frostii UFMGCB 1917
Diatrypellaelaeidis MFLUCC 15-0279 Diatrypella vulgaris HVFRA02
97/90
Diatrypella vulgaris HVGRF03 Paraeutypella guizhouensis KUMCC 20-0017 ail 1001 | Diatrypella heveae MFLUCC 17-0368 Diatrypella heveae MFLUCC 15-0274 Diatrypella atlantica HUEFS 136873 Diatrypella tectonae MFLUCC 12-0172a 0.98|' Diatrypella tectonae MFLUCC 12-0172b Diatrypella atlantica LCM 888.01 too | Diatrypella pseudooregonensis GMB0039 Diatrypella pseudooregonensis GMB0041
too | Diatrypella verruciformis UCROK754 ah = Diatrypella verruciformis UCROK1467
95/1
oon |Diatrype oregonensis CA117 Diatrype oregonensis DPL200
oon [Neoeutypella baoshanensis HMAS255436 omit Eutypella caricae EL51C
Diatrypella banksiae CPC29118
Allodiatrype taiyangheensis IFRDCC2800 Allodiatrype albelloscutata T1FRD9100 Allodiatrype trigemina FCATAS842 Allodiatrype elaeidicola MFLUCC 15-0737a ioo1 || Allodiatrype thailandica MFLU 15-3662 Allodiatrype elaeidis MFLUCC 15-0708a Allodiatrype arengae MFLUCC 15-0713 Stromatolinea xishuiensis GMB4535 10° | Stromatolinea xishuiensis GMB4522 09 ' Stromatolinea xishuiensis GMB4514
1oon| Stromatolinea guizhouensis GMB4523 -/0.99 Stromatolinea guizhouensis GMB4515
100/0.59 | Stromatolinea grisea GMB4512 0.91 Stromatolinea grisea GMB4508 Diatrypaceae sp. S21
100/1 = Stromatolinea hydei GMB4509 ioot|— Diatrypaceae sp. ZHKUCC_21-0114
Stromatolinea hydei GMB4538 094 Stromatolinea hydei GMB4521 100/1 Stromatolinea linearis MFLUCC 11-0503 Stromatolinea linearis MFLUCC 15-0198 78/098 Diatrype enteroxantha HUEFS 155114 100/0.99 |' Diatrype enteroxantha HUEFS 155116 Diatrype enteroxantha GMBC0433 Pseudodiatrype hainanensis GMBO055 Pseudodiatrype hainanensis GMB0054 Halodiatrype salinicola MFLUCC15-1277 Halodiatrype avicenniae MFLUCC15-0953 1001 | Pedumispora rhizophorae BCC44878 Pedumispora rhizophorae BCC44877
100/1
99/1
94/0.99
Diatrype palmicola MFLU 15-0040 Diatrype palmicola MFLU 15-0041
99/0.99
Figure 1. A-C. Phylogenetic tree generated from maximum likelihood analysis (RAXML) based on combined ITS and TUB2 sequences data. Bootstrap support values for maximum likelihood (ML) greater than 70% and Bayesian posterior probabilities (BPP) greater than 0.90 are displayed above or below the respective branches (ML/BPP). The species ob- tained in this study are in red and ex-type taxa are in bold.
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 204
Kamran Habib et al.: A new Diatrypaceous fungal genus
Diatrypella pulvinate H048 Diatrypella pulvinata DL29C Diatrypella yumnanensis VTO1 ‘Diatrypellahubeiensis CFCC52413 Diatrypellabetulicola CFCC52411 Diatrypella betulae CFCC52406 Diatrypella favacea DL26C Diatrypella favacea 380 Diatrype lancangensis GMB0046 Diatrype lancangensis GMB0045
98/0.99
-/0.94
-/0.97
85/0.97
100/1 72/0.91 Diatrypella guiyangensis GMBC0414
99/0.99
Diatrypella guiyangensis GMBC0415
Diatrypella fatsiae-japonica GMBC0423 Diatrypella fatsiae-japonica GMBC0422
toon Po Cryptosphaeria eunomia var. fraxini CSC Cryptosphaeria eunomia var. eunomia C1C
Eutypa lata var. acer CBS290.87 Eutypa laevata EAOC Eutypa lata EP18 Eutypa lata RGAO1 Eutypa armeniacae ATCC 28120 Eutypa camelliae HKAS 107022 Eutypa cerasi GMB0048
100/1 Eutypa cerasi GMB0049 too | Alloeutypa milinensis FCATAS 4382 Alloeutypa milinensis FCATAS4309_ Alloeutypa flavovirens EA8C
7910.98 100/1
85/0.99
-/0.90 92/0.99
70/- 81/0.97, 9710.99
Allocryptovalsa elaeidis MFLUCC 15-0707 Eutypella cryptovalsoidea HVFIGO2 Allocryptovalsa polyspora MFLUCC 17-0364
Allocryptovalsa sichuanensis HKAS 107017
80/0.97 100/1
Allocryptovalsa castaneae CFCC 52428 Allocryptovalsa xishuangbanica GMBC0417 Allocryptovalsa xishuangbanica KUMCC 21-0830 Cryptovalsa rabenhorstii WA08CB Cryptovalsa elevata CBS125574 Eutypella tamaricis MFLUCC 14-0445 Allocryptovalsa rabenhorstii GMBC0416 Eutypella microtheca BCMX01 toon) LUtypella citricola HVGRFO1 Eutypella citricola HVVIT07 Eutypella vitis UCD2428TX Eutypella vitis UCD2291AR Allocryptovalsa castaneicola CFCC 52432 Paraeutypella pseudoguizhouensis GMBC0421 Paraeutypella pseudoguizhouensis GMBC0420 Diatrypella longiasca KUMCC 20-0021 Eutypella leprosa 60 Eutypella leprosa EL54C
95/0.99
100/1 88/1
si
7iI-
93/1
98/1 -/0.97
99/1 95/1
88/493
81/0.97
100/0.99
Peroneutypa alsophila ELS58C Peroneutypa rubiformis MFLUCC 17-2142 Peroneutypa diminutiasca MFLUCC 17-2344 Eutypella curvispora HUEFS 136877 Peroneutypa kunmingensis HKAS 113189
76/0.97 81/0.99
88/0.99
75/0.96
75/0.99 -Peroneutypa longiasca MFLU 17-1217
Peroneutypa hainanensis GMBC0425
Peroneutypa hainanensis GMBC0424
100/1 -/0.91
Peroneutypa qianensis GMBC0432 Peroneutypa gianensis GMBC0431 Peroneutypa mackenziei MFLUCC 16-0072 Peroneutypa leucaenae MFLU 18-0816 Eutypa microasca BAFC51550 Peroneutypa polysporae NFCCI-4392 = Peroneutypa mangrovei PUFD526 Peroneutypa diminutispora HUEFS 192196
100/1
71/0.99
7110.99
99/1 99/0.99
Peroneutypa indica NFCCI-4393 Peroneutypa kochiana EL53M
78/0.99
Figure 1. Continued.
Taxonomy
Stromatolinea K. Habib & Q.R. Li, gen. nov. MycoBank No: 853267
Etymology. Referring to linear characteristics of the stromata.
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 205
Kamran Habib et al.: A new Diatrypaceous fungal genus
C
toon | Eutypella cerviculata ELS9C Eutypella cerviculata M68 Eutypella semicircularis MP4669 — Eutypella quercina TRAN 2543C ag, 1001| Zutypella motuoensis FCATAS 4082 Eutypella motuoensis FCAT AS 4085 Anthostoma decipiens 1PV-FW349 — Anthostoma decipiens JL567 Eutypella cearensis HUEFS 131070
100/1
95/0.99
86/1 100/1
| Vasilyeva cinnamomi GMBC0418 ! Vasilyeva cinnamomi GMBC0419 oon _[ Quaternaria quaternata GNF 13 ! Quaternaria quaternata EL60C
100/1
toon | Cryptovalsa ampelina DRO101
Cryptovalsa ampelina AO01
1001 ~~ Monosporascus cannonballus CMM3646 | Monosporascus cannonballus ATCC:26931
Eutypa maura CBS 219.87 i Eutypa sparsa 3802-3b
logis Eutypa lejoplaca CBS 248.87 Eutypa astroidea CBS 292.87 _
Halocryptovalsa salicorniae MFLUCC 15-0185
Eutypa leptoplaca CBS 287.87 » Eutypa tetragona CBS 284.87 san ml Cryptosphaeria subcutanea DSUB100A. 7210.99 ' Cryptosphaeria subcutanea CBS240.87 991 Cryptosphaeria ligniota CBS273.87
-10.96 ——————__ Cryptosphaeria pullmanensis ATCC 52655 i. Eutypella parasitica CBS 210.39 Diatrype bullata UCDDCh400 -0.99|'- Diatrype spilomea D17C Diatrype quercicola CFCC52418 Diatrypella macrospora KDQ15 Le Diatrypelta iranensis KDQ18 Irapso Diairype larissae FCATAS 2723 Diatrype betulaceicola FCATAS 2725 on | Diatrype disciformis GNA14 Diatrype disciformis D21C toon | Diatrype camelliaejaponicae GMBC0428 1oon| | Diatrype camelliaejaponicae GMBC0427 | Lo Diatrype rubi GMBC0430 Diatrype rubi GMBC0429 7510.95 Diatrype betulae GMBC0426
-/0.99
93/0.98
L095} "—Diatrype betulae CFCC 52416 '— Diatrype undulata D20C
7810.98 -— Diatrype castaneicola CFCC52425
-/0.98}
‘— Diatrype stigma DCASH200 Diatrype lijiangensis MFLU 19-0717
Figure 1. Continued.
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712
100 L
Xylaria hypoxylon CBS122620 Kretzschmaria deusta CBS 826.72
Type species. Stromatolinea hydei K. Habib & Q.R. Li, sp. nov.
Description. Saprobic on dead bamboo culms, forming black parallel elongate ascostromata on the host, surrounded by grey or black patches like pseudostro- mata. Pseudostromata grey or black, spreading between stromata and across the host surface. Sexual morph: Stromata parallel elongate, linear, consistent to inconsistent in thickness, fusiform, high, solitary to confluent, slit to non-slit, black or grey on its sides, exposing black ostioles. Upper cells of stromata near the perithecial ostiole black, thick-walled. Stromatic tissue completely yellow or yel- low above and white between/below perithecia, compact. Ascomata perithecial, few to frequent, immersed in stromata, globose to subglobose, ostiolate centrally, with a neck, opening to outer surface, slight erumpent over stromata, appearing as black shinny spots. Peridium composed of elongate cell, texture angularis, out- er thick layer, dark brown, inner hyaline, surrounded by yellow or white and yellow stromatic tissue. Hamathecium paraphyses, filiform, hyaline, long. Asci 8-spored, clavate, with a long and thin pedicel, apically rounded to truncate, J- apical ring. Ascospores allantoid, aseptate, straight to slightly curved, rounded at both ends, subhyaline, with oil droplets in both ends. Asexual morph: undetermined.
Notes. Phylogenetically, Eutypa is polyphyletic (Fig. 1), a species distributed in different clades. Ma et al. (2023) proposed a new genus, Alloeutypa, which exhibits close affinity to Eutypa. However, based on the presence of Diatrype-like
206
Kamran Habib et al.: Anew Diatrypaceous fungal genus
discrete stromata with yellowish-green interior tissue characteristics and form- ing separate monophyletic clades, they proposed Alloeutypa as a new genus to accommodate Alloeutypa milinensis and A. flavovirens (Ma et al. 2023).
Morphologically, Stromatolinea is similar to Alloeutypa, as both possess yellowish-green interior tissue. However, the new genus is distinguished from Alloeutypa by its linear stromata and phylogenetically, they are clustered very distantly. The strains of Stromatolinea form a monophyletic clade representing its distinct position. Thus, based on morphological evidence and phylogenetic analyses, we accommodate Stromatolinea as a new genus with Stromatolinea hydei designated as the type species.
Stromatolinea grisea K. Habib & Q.R. Li, sp. nov. MycoBank No: 853270 Fig. 2
Type. * CHINA, Guizhou Province, Xishui Country, Changjian Gully, (28°19'58'N, 106°11'50"E), altitude: 1,180 m, subtropical forest, on dead culms of Phyl- lostachys sp., 27 December 2023, Xin Zhou, R-17, (Holotype, GMB4512; ex-type, GMBC4512; isotype, KUN-HKAS133213).
Etymology. The epithet “grisea” refers to the grey color of pseudostromata.
Description. Saprobic on dead culms of Phyllostachys sp., forming black paral- lel elongate ascostromata on the host, surrounded by grey patches like pseudos- tromata. Pseudostromata grey, spreading between stromata and across the host surface. Sexual morph: Stromata 2-15 mm long, 400-800 um wide, 400-600 um high, parallel elongate, inconsistent in thickness, thin in between, fusiform, solitary to confluent, non-slit, black, exposing black ostioles. Upper cells of stromata near the perithecial ostiole black, thick-walled. Stromatic tissue yellow above and white between/below perithecia, compact. Ascomata 250-420 um wide, 260-450 um high, perithecial, 2-5 per stromata, usually 2 or 3 per stromata, immersed in stro- mata, globose to subglobose, ostiolate centrally, with a neck, opening to outer surface, 80-100 x 35-60 um, slight erumpent over stromata, appearing as black shinny spots. Peridium 15-30 pm thick, cell elongate, texture angularis, outer thick layer dark brown, inner hyaline, surrounded by yellow stromatic tissue. Hamathe- cium paraphyses, filiform hyaline, 50-73 x 1-3.2 um. Asci 50-95 x 5.5-9.8 um (x = 73 x 6.2 um, n = 30), 8-spored, unitunicate, clavate, with a long and thin pedicel, apically rounded to truncate, J- apical ring. Ascospores 5.8-8.2 x 1.4-2 um (x = 7.5 x 1.6 um, n = 30), allantoid, aseptate, straight to slightly curved, rounded at both ends, subhyaline, single oil droplets in both ends. Asexual morph: Undetermined.
Culture characteristics. Ascospores germinating on PDA within 24 hours, colonies on PDA, white when young, became pale, thinning toward the edge, white from above, reverse pale, no pigmentation, and no sporulation produced on the PDA medium.
Addition material examined. * CHINA, Guizhou Province, Xishui Country, Chang- jian Gully, (28°19'56'N, 106°11'48"E), altitude: 1,180 m, subtropical forest, on dead culms of Phyllostachys sp., 2 January 2024, Lili Liu & Yan Lin, R-200 (GMB4508).
Notes. It is morphologically similar to Stromatolinea hydei and S. xishuiensis but can be easily distinguished by the appearance of stromata and pseudostro- mata color. The pseudostromata of the latter two species are black, whereas grey
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 207
Kamran Habib et al.: Anew Diatrypaceous fungal genus
h Tai
Figure 2. Stromatolinea grisea (GMB4512) a habitat of a type material b-e appearance of stromata and pseudostromata on bamboo host f peridium of ascoma g horizontal section of ascostromata h, i asci j vertical sections of ascomata in stroma k ascospores. Scale bars: 1 mm (b); 3 mm (c-e); 30 um (f); 0.4 mm (g, j); 20 um (h, i); 10 pm (k).
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 208
Kamran Habib et al.: A new Diatrypaceous fungal genus
in S. grisea. The stromata of S. hydei and S. xishuiensis are consistent in thickness and possess frequent ascomata, whereas the stromata of S. grisea are inconsis- tent in thickness, thin in between, and possess usually 2 or 3 ascomata. Moreover, stromatic tissue is yellow above and white between or below perithecia in S. grisea, while it is completely yellow in S. hydei and S. xishuiensis. The comparison of ITS sequences revealed 94% and 98% similarity to S. hydei and S. xishuiensis, respec- tively, while TUB2 sequences displayed 93% and 94% similarity to S. hydei and S. xishuiensis, respectively. Differentiation from other known species of the genus is discussed in the note section of the below described species.
Stromatolinea guizhouensis K. Habib & Q.R. Li, sp. nov. MycoBank No: 853272 Fig. 3
Type. * CHINA, Guizhou Province, Anshun City, Pingba County (26°15'11'N, 105°56'51"E), altitude: 1,102 m, subtropical forest, on dead culms of Phyl- lostachys sp., 25 August 2023, JWS-28 (Holotype, GMB4523; ex-type, GMBC4523; isotype, KUN-HKAS133214).
Etymology. The epithet “guizhouensis” refers to the locality of the collection, Guizhou province.
Description. Saprobic on dead culms of Phyllostachys sp., forming black parallel elongate ascostromata on the host. Pseudostromata absent. Sexual morph: Stromata 2-8.5 mm long, 350-800 um wide, 400-600 um high, par- allel elongate, consistent in thickness, linear, long fusiform, solitary, sometime confluent, non-slit, distinctly gray at sides, often overlain by white crystalline, black at center, exposing black ostioles. Upper cells of stromata near the perith- ecial ostiole black, thick-walled. Stromatic tissue yellow between and beneath perithecia, compact. Ascomata 150-250 um wide, 250-420 um high, perithe- cial, frequent, 10-25 per stromata, immersed, linearly arranged, obpyriform, os- tiolate centrally, with a neck, opening to outer surface, 70-90 x 35-60 um, slight erumpent over stromata, appearing as black spots, slight shinny. Peridium 5-10 um thick, cell elongate, texture angularis, outer thick layer dark brown, inner hya- line, surrounded by yellow stromatic tissue. Hamathecium paraphyses, filiform, hyaline, 54-70 x 1-3.2 um. Asci 55-100 x 5.5-8 um (x = 67.2 x 6.8 um, n = 30), 8-spored, unitunicate, clavate, with a long and thin pedicel, apically rounded to truncate, J- apical ring. Ascospores 5.8-9 x 1-2 um (x = 7.6 x 1.5 um, n = 30), allantoid, aseptate, straight to slightly curved, rounded at both ends, subhyaline, smooth-walled, single oil droplets in both ends. Asexual morph: Undetermined.
Culture characteristics. Ascospores germinating on PDA within 24 hours, colonies on PDA, white when young, became pale, dense at centre, thinning to- ward the edge, reverse white at the margin, pale at the centre, no pigmentation, and no sporulation produced on the PDA medium.
Additional material examined. * CHINA, Guizhou Province, Huaxi District, Xiaohe Village, China (26°29'29"N, 106°42'09"E), altitude: 1,097 m, subtropical forest, on dead culms of Phyllostachys sp., 2 January 2024, Xin Zhou & W.Y. Zeng, H-8 (GMB4515).
Notes. Morphologically, Stromatolinea guizhouensis is similar to Stromat- olinea linearis (= Diatrype phaselinoides Rappaz; Eutypa linearis Rehm), both
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 209
Kamran Habib et al.: A new Diatrypaceous fungal genus
a
g | h Figure 3. Stromatolinea guizhouensis (GMB4523) a habitat of material b, c appearance of stromata on host d erumpent ostiole e horizontal section of ascostromata f vertical sections of ascomata in stroma g-i asci j apical ring of ascus
k ascospores. Scale bars: 3 mm (b, c); 1 mm (d-f); 20 um (g-j); 10 pm (k).
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 210
Kamran Habib et al.: Anew Diatrypaceous fungal genus
exhibiting parallel elongate fusiform stromata with yellow stromatic tissue. However, it differs from S. linearis in having non-slit stromata, distinctly grey at sides, overlain by white crystalline material (Fig. 3b, c) and slightly larger asco- spores (5.8-9 um, xX = 7.6 um), compared to S. linearis with longitudinally slit stromata when mature and smaller ascospores (5-7 um, x = 6.1 um) (Rehm 1907; Dai et al. 2017). From the other newly described species, it lacks pseu- dostromata and exhibits grey stromata overlain by white crystalline material.
Another morphologically similar species, Alloeutypa milinensis also features yellow stromatic tissue but can be easily differentiated by its stromata mor- phology. Alloeutypa milinensis exhibits scattered oblong to strip-shaped stro- mata measuring 0.9-2.2 mm in width and with larger ascospores (6.6-10.1 x 1.7-2.6 um, x = 8.5 x 2.1 um) (Ma et al. 2023).
Stromatolinea hydei K. Habib & Q.R. Li, sp. nov. MycoBank No: 853274 Fig. 4
Type. * CHINA, Guizhou Province, Anlong County Suburban (25°05'56'N, 105°26'34"E), altitude: 856 m, subtropical forest, on dead culms of Phyl- lostachys sp., 23 September 2023, Youpeng Wu, JWS-8 (Holotype, GMB4509; ex-type, GMBC4509; isotype, KUN-HKAS133215).
Etymology. The epithet “hydei” pays tribute to the renowned mycologist, Prof. Kevin David Hyde, in recognition of his valuable contributions to the field of mycology.
Description. Saprobic on dead culms of Phyllostachys sp., forming black parallel elongate ascostromata on the host, surrounded by black patches like pseudostromata. Pseudostromata black, spreading between stroma- ta and across the host surface forming the darkened region. Sexual morph: Stromata 2-10 mm long, 400-800 um wide, 400-620 um high, parallel elon- gate, straight, long fusiform, solitary, sometime confluent, slit when mature, black, above plane, exposing black ostioles. Upper cells of stromata near the perithecial ostiole black, thick-walled. Stromatic tissue yellow between and beneath perithecia, compact. Ascomata 150-270 um wide, 260-440 um high, perithecial, frequent, 10-25 per stromata, immersed in stromata, linearly ar- ranged, obpyriform, ostiolate centrally, with a neck, opening to outer surface, 80-100 x 35-60 um, slight erumpent over stromata, appearing as black spots, slight shinny. Peridium 5-10 um thick, cell elongate, texture angularis, outer thick layer dark brown, inner hyaline, surrounded by yellow stromatic tissue. Hamathecium paraphyses, filiform. hyaline, 50-68 x 1-3.5 um. Asci 50-80 x 5.5-8 um (x = 64 x 6.5 um, n = 15), 8-spored, unitunicate, clavate, with a long and thin pedicel, apically rounded to truncate, a J- apical ring. Ascospores 5.8-10 x 1.4-2.5 um (x = 8 x 1.8 um, n = 20), allantoid, aseptate, straight to slightly curved, rounded at both ends, subhyaline, single oil droplets in both ends. Asexual morph: Undetermined.
Culture characteristics. Ascospores germinating on PDA within 24 hours, colonies on PDA, white when young, became pale, dense at centre, thinning toward the edge, reverse pale-white, no pigmentation, and no sporulation pro- duced on the PDA medium.
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 11
Kamran Habib et al.: A new Diatrypaceous fungal genus
F - \ ° e 8 * ' * h I J J ae 1) aoe Figure 4. Stromatolinea hydei (GMB4509) a habitat of material b, c appearance of stromata on host d vertical section of ascomata in stroma e horizontal section of ascostromata f vertical section of ascoma g-j asci k-m ascospores. Scale
bars: 3 mm (b, c); 0.5 mm (d, e); 100 um (f); 15 um (g-j); 5 um (k-m).
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 912
Kamran Habib et al.: A new Diatrypaceous fungal genus
Addition material examined. CHINA * Guizhou Province, Xishui Country, Changjian Gully, (28°19'57"N, 106°11'32"E), altitude: 1,180 m, subtropical for- est, on dead culms of Phyllostachys sp., 2 January 2024, Lili Liu & Yan Lin, R-27 (GMB4538) * CHINA, Guizhou Province, Xishui Country, Changjian Gully, (28°19'51"N, 106°11'49"E), altitude: 1,185 m, subtropical forest, on dead culms of Phyllostachys sp., 2 January 2024, Xin Zhou, R-4 (GMB4521).
Notes. Stromatolinea hydei is morphologically similar to S. linearis (=Diatrype phaselinoides; Eutypa linearis), both displaying parallel elongate fusiform stro- mata with yellow stromatic tissue and slit mature stromata. However, S. hydei is distinguishable by its stromata, which are surrounded by black patches re- sembling pseudostromata, which spread between stromata and across the host surface, forming a darkened region. Additionally, S. hydei has larger as- cospores 5.8-10 x 1.4—2.5 um (with an average of 8 x 1.8 um), compared to S. linearis, whose ascospores range from 5-7 x 1-2 um, with an average of 6.1 x 1.4 um (Dai et al. 2017).
Stromatolinea xishuiensis K. Habib & Q.R. Li, sp. nov. MycoBank No: 853275 Fig. 5
Type. * CHINA, Guizhou Province, Xishui Country, Changjian Gully, (28°19'58'N, 106°11'50"E), altitude: 1,180 m, subtropical forest, on dead culms of Phyl- lostachys sp., 27 December 2023, Xin Zhou, R-7 (Holotype, GMB4535; ex-type, GMBC4535; isotype, KUN-HKAS133216).
Etymology. The epithet “xishuiensis” refers to the locality of the collection, Xishui County.
Description. Saprobic on dead bamboo culms, forming black parallel elon- gate ascostromata on the host, surrounded by black patches like pseudostro- mata. Pseudostromata black, spreading between stromata and across the host surface forming the darkened region. Sexual morph: Stromata 2-10 mm long, 350-600 um wide, 400-550 um high, parallel elongate, consistent in thickness, straight, long fusiform, solitary to confluent, non-slit, black, exposing black os- tioles. Upper cells of stromata near the perithecial ostiole black, thick-walled. Stromatic tissue yellow between and beneath perithecia, compact. Ascomata perithecial, 150-220 um wide, 240-300 um high, frequent, 10-28 per stromata, immersed in stromata, obpyriform, irregular arranged, ostiolate centrally, with a neck, opening to outer surface, slight erumpent over stromata, appearing as black spots. Peridium 5-15 um thick, cell elongate, texture angularis, outer thick layer dark brown, inner hyaline, surrounded by yellow stromatic tissue. Hamath- ecium paraphyses, hyaline, 50-70 x 1-3.6 um, filiform. Asci 60-90 x 5.5-8 um (x = 70 x 7 um, n = 15), 8-spored, clavate, with a long and thin pedicel, apically rounded to truncate, J- apical ring. Ascospores 5.8-8.2 x 1—2.2 um (x = 7.1 x 1.4 um, n = 20), allantoid, aseptate, straight to slightly curved, rounded at both ends, subhyaline, 1-2 oil droplets in both ends. Asexual morph: Undetermined.
Culture characteristics. Ascospores germinating on PDA within 24 hours, colonies on PDA, white when young, pale and dense at centre, thinning toward the edge, reverse pale-white, no pigmentation, and no sporulation produced on the PDA medium.
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 213
Kamran Habib et al.: Anew Diatrypaceous fungal genus
Figure 5. Stromatolinea xishuiensis (GMB4535) a habitat of material b-d appearance of stromata on host e vertical sec- tion of ascomata in stroma f horizontal section of ascostromata g, h asci i ascospores. Scale bars: 3 mm (b, c); 1 mm
(d); 0.5 mm (e, f); 20 um (g, h); 10 um (i).
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 214
Kamran Habib et al.: Anew Diatrypaceous fungal genus
Additional material examined. * CHINA, Guizhou Province, Zunyi City, Suiyang Country, Kuankuoshui National Nature Reserve (28°29'33.64"N, 107°9'23.66'E), altitude: 1,634 m, subtropical forest, on dead culms of Phyllostachys sp., 23 September 2023, Qirui Li, K3N (GMB4522) + CHINA, Guizhou Province, Zunyi City, Xishui Country, Changjian Gully, (28°19'58"N, 106°11'54"E), altitude: 1,180 m, on dead culms of Phyllostachys sp., 27 December 2023, Xin Zhou, R-5, (GMB4514).
Notes. Stromatolinea xishuiensis can be distinguished from S. guizhouensis and S. linearis by its stromata surrounded by black patches spread between the stromata and across the host surface, forming a darkened region. Moreover, its ascomata are irregularly arranged in stroma. Morphologically, it is most similar to S. hydei, which also exhibits black patches spreading between stromata and across the host surface. However, S. hydei has slightly wider stromata, measuring 400-800 um wide and 400-620 um high, linearly arranged larger ascomata, mea- suring 150-270 um wide and 260-440 um high, and bigger ascospores, measur- ing 5.8-10 x 1.4-2.5 um (average 8 x 1.8 um). The ITS and B-tubulin sequence data of S. xishuiensis and S. hydei demonstrates 94% and 95% similarity, respectively.
Stromatolinea linearis (Rehm) K. Habib & Q. R. Li, comb. nov. MycoBank No: 853282
Eutypa linearis Rehm, Annls mycol. 5(6): 523 (1907) (Basionym). Synonym = Diatrype phaselinoides Rappaz, Mycol. helv. 2(3): 442 (1987). Synonym
Description. See Dai et al. (2017).
Notes. The fungus was originally documented by Rehm (1907) from a speci- men collected in Brazil, Eutypa linearis underwent a taxonomic revision by Rap- paz (1987), who reclassified it as Diatrype phaselinoides (non Diatrype linearis Ellis & Everh. 1897). Dai et al. (2017) provided molecular data and a reference specimen of this taxon. It is characterized by well-developed linear stromata with yellow-green interior tissue, long-stipitate asci, with hyaline to subhyaline allantoid ascospores, measuring 5-7 x 1.5-1.8 um (Rappaz 1987; Dai et al. 2017). Phylogenetically, it clusters together with other Stromatolinea species in a distinct clade. The morphological character of this taxon also aligns with those of Stromatolinea, providing compelling support for its placement within the Stromatolinea taxonomic framework.
Stromatolinea phaselina (Mont.) K. Habib & Q. R. Li, comb. nov. MycoBank No: 855036
Sphaeria phaselina Mont., Ann. Sci. Nat., Bot., sér. 4 3: 129 (1855) (Basionym). Synonym. = Diatrype phaselina (Mont.) Rappaz, Mycol. Helv. 2(3): 442 (1987). Synonym."
Notes. Stromatolinea phaselina was first described and illustrated by Montagne in 1855 based on a collection from Guyana. Rappaz (1987) conducted a de-
1 See Rappaz (1987) for other synonyms and description.
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 215
Kamran Habib et al.: Anew Diatrypaceous fungal genus
tailed analysis of various species that had been reported under different names (given above as synonyms). He reviewed their descriptions and type materials, though some of type material were lost. Rappaz (1987) synonymized E. kusanoi and E. bambusina with Eutypa hypoxantha and grouped these along with Sphae- ria phaselina and Eutypella hypoxantha under the broader name Diatrype phase- lina (Mont.) Rappaz. The morphological character of this taxon also aligns with those of Stromatolinea, providing compelling support for its placement within the Stromatolinea taxonomic framework.
Morphologically, Stromatolinea phaselina resembles S. grisea in having yel- low entostromatic tissue above and white tissue between or below the perith- ecia. However, there are no reports of pseudostromata presence, detailed stro- mata morphology, or the number of perithecia per stromata in the published description of Diatrype phaselina (Rappaz 1987). Furthermore, descriptions and synonymized accounts of this species report very short asci sizes, mea- suring 25-35 x 5-7 um. This is problematic because the family is known to typically possess long asci. This gap in detailed morphological data limits our ability to fully understand and differentiate Diatrype phaselina from other spe- cies. Without a detailed description and access to type material or DNA data, we cannot definitively classify it within the key of the genus.
The synonyms of this species are not updated in Index Fungorum and Myco- bank, where they are still listed as separate species. Given Rappaz (1987) thor- ough analysis of historical descriptions and most of the original materials, we con- sider his classification/synonyms of the species to be well-founded and reliable.
Discussion
The generic concepts of Diatrypaceae have been unstable; several new genera within the family have been reported through a combination of morphological characteristics and multi-locus phylogeny. Early classification systems of Di- atrypaceae were mainly based on stromatal features including the degree of stromatal development, structure of perithecial necks, and type of host tissue (Glawe and Jacobs 1987; Rappaz 1987). However, the morphological variability of stromata has caused significant confusion within Diatrypaceae. Many genera, including Neoeutypella, Allodiatrype, Diatrype, Diatrypella, Allocryptovalsa, Cryp- tovalsa, Eutypella, and Paraeutypella, exhibit similar stromatal characteristics, limiting their utility for species identification (Li et al. 2023). This confusion has led to polyphyletic genera, where species have often been transferred between genera (Shang et al. 2017, 2018; Phookamsak et al. 2019; Konta et al. 2020; Ma et al. 2023). In this study, we introduce a new genus that phylogenetically forms a well-supported distinct clade and morphologically distinguished by its linear stro- mata and yellow interior tissue. This new genus includes four new species name- ly S. grisea, S. guizhouensis, S. hydei, and S. xishuiensis. Additionally, Stromato- linea linearis and S. phaselina are proposed for Eutypa linearis and D. phaselina, respectively, based on morphological characteristics and comparative analysis. Species within Stromatolinea can be differentiated by key morphological features, including the presence or absence and color of pseudostromata; stromata size, color, slit presence, and interior tissue color; ascomata number, arrangement, and measurements; and ascospores dimensions. Furthermore, significant phylogenetic distances in the ITS and TUB2 regions also serve as
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 216
Kamran Habib et al.: Anew Diatrypaceous fungal genus
valuable tools for species discrimination. Notably, all Stromatolinea species have been reported as saprobes on dead bamboo, implying a potential host specificity confined to bamboo.
Key to species
1 PSCUGESIIGMalaraly SOM Sf... Ay eceane by Woda noson tae ute A rcarae beeline, mepebtah 2 - Pseudostromata well-developed.................cccccccssscsccesssnecessoescessseesnesetsccesens 3 2 non-slit stromata, distinctly grey at sides, ascospores 5.8-9 um long, av- STAGING: = 7. O PN lagrcdeccercsncernh aca yecdtoneln clan anencekeurinvece 4 ateepeetexns S. guizhouensis - stromata slit when mature, color black, ascospores 5-7 um long, averag- LI = y1 HMA eect ee cbcadeas tees ee. cee easnca ee eg OnE Socata teas S. linearis
3. grey pseudostromata, stromata inconsistent in thickness, possess few as- comata, interior tissue yellow above and white between or below perithecia, ascospores 5.8-8.2 x 1.4—2 um, averaging = 7.5 x 1.6 um.............. S. grisea
- black pseudostromata, stromata consistent in thickness ..................08 a
4 stromata 400-800 um wide, slit when mature, ascomata linearly ar- ranged, 150-270 um wide and 260-440 um high, ascospores 5.8-10 x 142M AVEAGING HB e% TBs MIM rnc cscds cuca ssavcconsntaccnmaenadd ar ccordasd ceees S. hydei
- stromata 350-600 um wide, non-slit, ascomata irregularly arranged, 150- 220 um wide and 240-300 um high, ascospores 5.8-8.2 x 1-2.2 um, AVETA GING =<75 NSE AL LMM avhds Prd veseeest nnvhvccesneenrbtiaetandanenath S. xishuiensis
Acknowledgements
This research was supported by Guizhou Medical University High level Talent Launch Fund Project (No. [2023] 058); the National Natural Science Foundation of China (31960005, 32170019 and 31960716); the Guizhou Provincial Scientif- ic and Technologic Innovation Base (No. [2023] 003); the High-level Innovation Talents of Guizhou (No. GCC [2023] 048); National Natural Science Foundation of China (12132006); the Guizhou Provincial Natural Science Foundation for High-Level Innovative Talents and Teams (2016-5676, 2015-4021).
Additional information
Conflict of interest
The authors have declared that no competing interests exist.
Ethical statement
No ethical statement was reported.
Funding
This research received no specific grant from any funding agency in the public, commer- cial, or not-for-profit sectors.
Author contributions
Conceptualization: Kamran Habib, Jichuan Kang, Qirui Li. Collection and morphological examinations: Xin Zhou, Wenyu Zen, Xu Zhang, Lili Liu, Yan Lin and Hongmin Hu. Molecular sequencing, and phylogenetic analyses: Hongmin Hu,
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 917
Kamran Habib et al.: A new Diatrypaceous fungal genus
Kamran Habib and Qianzhen Wu. Specimen identification: Kamran Habib, Qirui Li. Original draft preparation: Kamran Habib, Qirui Li. Review and editing, supervision: Xiangchun Shen and Jichuan Kang. All authors have read and agreed to the published version of the manuscript.
Author ORCIDs
Kamran Habib © https://orcid.org/0000-0003-2572-0306 Hongmin Hu © https://orcid.org/0000-0003-3894-3269 Qianzhen Wu ® https://orcid.org/0000-0001-9992-8404 Jichuan Kang ® https://orcid.org/0000-0002-6294-5793 Qirui Li © https://orcid.org/0000-0001-8735-2890
Data availability
The datasets generated during and/or analyzed during the current study are available in the MycoBank repository (included in the manuscript), and GenBank (included in Table 1). And also, the datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
References
Acero FJ, Gonzalez V, Sanchez-Ballesteros J, Rubio V, Checa J, Bills GF, Salazar O, Platas G, Pelaez F (2004) Molecular phylogenetic studies on the Diatrypaceae based on rDNA-ITS sequences. Mycologia 96(2): 249-259. https://doi.org/10.1080/1557253 6.2005.11832975
Arhipova N, Gaitnieks T, Donis J, Stenlid J, Vasaitis R (2012) Heart-rot and associated fungi in Alnus glutinosa stands in Latvia. Scandinavian Journal of Forest Research 27(4): 327-336. https://doi.org/10.1080/02827581.2012.670727
Carmaran CC, Romero Al, Giussani LM (2006) An approach towards a new phylogenetic classification in Diatrypaceae. Fungal Diversity 23: 67-87.
Chen YP, Su PW, Hyde KD, Maharachchikumbura SSN (2023) Phylogenomics and diver- sification of Sordariomycetes. Mycosphere 14(1): 414-451. https://doi.org/10.5943/ mycosphere/14/1/5
Chomnunti P Hongsanan S, Aguirre-Hudson B, Tian Q, Persoh D, Dhami KM, Alias AS, Xu J, Liu X, Stadler M, Hyde KD (2014) The sooty moulds. Fungal Diversity 66(1): 1-36. https://doi.org/10.1007/s13225-014-0278-5
Crous PW, Wingfield MJ, Burgess TI, Hardy GS, Crane C, Barrett S, Cano-Lira JF, Le Roux JJ, Thangavel R, Guarro J, Stchigel AM (2016) Fungal planet description sheets: 469- 557. Persoonia — Molecular Phylogeny and Evolution of Fungi 37: 218-403. https:// doi.org/10.3767/003158516X694499
Dai DQ, Phookamsak R, Wijayawardene NN, Li WJ, Bhat DJ, Xu JC, Taylor JE, Hyde KD, Chukeatirote E (2017) Bambusicolous fungi. Fungal Diversity 82(1): 1-105. https:// doi.org/10.1007/s13225-016-0367-8
Dai DQ, Tang LZ, Wang HB (2018) A review of bambusicolous ascomycetes. Bamboo —- current and future prospects. IntechOpen, 165-183. https://doi.org/10.5772/ intechopen. 76463
Dayarathne MC, Phookamsak R, Hyde KD, Manawasinghe IS, Toanun C, Jones EBG (2016) Halodiatrype, a novel diatrypaceous genus from mangroves with H. salinicola and H. avicenniae spp. nov. Mycosphere: Journal of Fungal Biology 7(5): 612-627. https://doi.org/10.5943/mycosphere/7/5/7
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 218
Kamran Habib et al.: Anew Diatrypaceous fungal genus
Dayarathne MC, Jones EB, Maharachchikumbura SS, Devadatha B, Sarma VV, Khongphi- nitbunjong K, Chomnunti P Hyde KD (2020a) Morpho-molecular characterization of microfungi associated with marine based habitats. Mycosphere: Journal of Fungal Biology 11(1): 1-188. https://doi.org/10.5943/mycosphere/11/1/1
Dayarathne MC, Wanasinghe DN, Devadatha B, Abeywickrama P Jones EG, Chomnunti P Sarma VV, Hyde KD, Lumyong S, Mckenzie EH (2020b) Modern taxonomic approaches to identifying diatrypaceous fungi from marine habitats, with a novel genus Halocryptovalsa Dayarathne and K.D. Hyde, gen. nov. Cryptogamie. Mycologie 41(3): 21-67. https://doi.org/10.5252/cryptogamie-mycologie2020v41a3
de Almeida DAC, Gusmao LFP, Miller AN (2016) Taxonomy and molecular phylogeny of Diatrypaceae (Ascomycota, Xylariales) species from the Brazilian semi-arid re- gion, including four new species. Mycological Progress 15(6): 1-27. https://doi. org/10.1007/s11557-016-1194-8
Dissanayake LS, Wijayawardene NN, Dayarathne MC, Samarakoon MC, Dai DQ, Hyde KD, Kang JC (2021) Paraeutypella guizhouensis gen. et sp. nov. and Diatrypella longiasca sp. nov. (Diatrypaceae) from China. Biodiversity Data Journal 9: e63864. https://doi. org/10.3897/BDJ.9.e63864
Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes - application to the identification of mycorrhizae and rusts. Molecular Ecology 2(2): 113-118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
Glass NL, Donaldson GC (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Envi- ronmental Microbiology 61(4): 1323-1330. https://doi.org/10.1128/aem.61.4.1323- 1330.1995
Glawe DA, Jacobs KA (1987) Taxonomic notes on Eutypella vitis, Cryptosphaeria populina, and Diatrype stigma. Mycologia 79(1): 135-139. https://doi.org/10.1080/ 00275514.1987.12025379
Grassi E, Belen Pildain M, Levin L, Carmaran C (2014) Studies in Diatrypaceae: The new species Eutypa microasca and investigation of ligninolytic enzyme production. Sydowia 66: 99-114.
Hall TA (1999) BioEdit: A user-friendly biological sequence alignment editor and anal- ysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95-98. Hyde KD, Tennakoon DS, Jeewon R, Bhat DJ, Maharachchikumbura SS, Rossi W, Leonar- di M, Lee HB, Mun HY, Houbraken J, Nguyen TT, Jeon SJ, Frisvad JC, Wanasinghe DN, Licking R, Aptroot A, Caceres MES, Karunarathna SC, Hongsanan S, Phookamsak R, de Silva NI, Thambugala KM, Jayawardena RS, Senanayake IC, Boonmee S, Chen J, Luo Z-L, Phukhamsakda C, Pereira OL, Abreu VP Rosado AWC, Bart B, Randrianjoha- ny E, Hofstetter V, Gibertoni TB, Soares AMS, Plautz Jr HL, Sotao HMP Xavier WKS, Bezerra JDP de Oliveira TGL, de Souza-Motta CM, Magalhaes OMC, Bundhun D, Har- ishchandra D, Manawasinghe IS, Dong W, Zhang S-N, Bao D-F, Samarakoon MC, Pem D, Karunarathna A, Lin C-G, Yang J, Perera RH, Kumar V, Huang S-K, Dayarathne MC, Ekanayaka AH, Jayasiri SC, Xiao Y, Konta S, Niskanen T, Liimatainen K, Dai Y-C, Ji X-H, Tian X-M, Mesié A, Singh SK, Phutthacharoen K, Cai L, Sorvongxay T, Thiyagaraja V, Norphanphoun C, Chaiwan N, Lu Y-Z, Jiang H-B, Zhang J-F, Abeywickrama PD, Aluth- muhandiram JVS, Brahmanage RS, Zeng M, Chethana T, Wei D, Réblova M, Fournier J, Nekvindova J, do Nascimento Barbosa R, dos Santos JEF, de Oliveira NT, Li G-J, Ertz D, Shang Q-J, Phillips AJL, Kuo C-H, Camporesi E, Bulgakov TS, Lumyong S, Jones EBG, Chomnunti P Gentekaki E, Bungartz F, Zeng X-Y, Fryar S, Tkalcec Z, Liang J, Li G, Wen T-C, Singh PN, Gafforov Y, Promputtha |, Yasanthika E, Goonasekara ID, Zhao R-L,
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 219
Kamran Habib et al.: Anew Diatrypaceous fungal genus
Zhao Q, Kirk PM, Liu J-K, Yan JY, Mortimer PE, Xu J, Doilom M (2019) Fungal diversity notes 1036-1150: Taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity 96(1): 1-242. https://doi.org/10.1007/s13225-019- 00429-2
Hyde KD, Norphanphoun C, Maharachchikumbura SS, Bhat DJ, Jones E, Bundhun D, Chen YJ, Bao DF, Boonmee S, Calabon MS, Chaiwan N (2020) Refined families of Sordariomycetes. Mycosphere: Journal of Fungal Biology 11(1): 305-1059. https:// doi.org/10.5943/mycosphere/11/1/7
Hyde KD, Norphanphoun C, Ma J, Yang HD, Zhang JY, Du TY, Gao Y, Gomes de Farias AR, He SC, He YK, Li CJY, Li JY, Liu XF, Lu L, Su HL, Tang X, Tian XG, Wang SY, Wei DP Xu RF, Xu RJ, Yang YY, Zhang F, Zhang Q, Bahkali AH, Boonmee S, Chethana KWT, Jayawardena RS, Lu YZ, Karunarathna SC, Tibpromma S, Wang Y, Zhao Q (2023) Mycosphere notes 387-412 — novel species of fungal taxa from around the world. Mycosphere: Journal of Fungal Biology 14(1): 663-744. https://doi.org/10.5943/my- cosphere/14/1/8
Jiang H, Phookamsak R, Hongsanan S, Bhat DJ, Mortimer PE, Suwannarach N, Kakumyan P. Xu J (2022) A review of bambusicolous Ascomycota in China with an emphasis on species richness in southwest China. Studies in Fungi 7(1): 20. https:// doi.org/10.48130/SIF-2022-0020
Jure D, Ogris N, Slippers B, Stenlid J (2006) First report of Eutypella canker of Acer pseudoplatanus in Europe. Plant Pathology 55(4): 577-577. https://doi.org/10.1111/ j.1365-3059.2006.01426.x
Katoh K, Rozewicki J, Yamada KD (2019) MAFFT online service: Multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20(4): 1160-1166. https://doi.org/10.1093/bib/bbx108
Klaysuban A, Sakayaroj J, Jones EG (2014) An additional marine fungal lineage in the Diatrypaceae, Xylariales: Pedumispora rhizophorae. Botanica Marina 57(5): 413-420. https://doi.org/10.1515/bot-2014-0017
Konta S, Maharachchikumbura SSN, Senanayake IC, Mckenzie EHC, Stadler M, Boonmee S, Phookamsak R, Jayawardena R, Senwanna C, Hyde KD, Elgorban AM, Eungwanichayapant PD (2020) A new genus Allodiatrype, five new species and a new host record of diatrypaceous fungi from palms (Arecaceae). Mycosphere: Journal of Fungal Biology 11(1): 239-268. https://doi.org/10.5943/mycosphere/11/1/4
Li GJ, Hyde KD, Zhao RL, Hongsanan S, Abdel-Aziz FA, Abdel-Wahab MA, Alvarado P Alves-Silva G, Ammirati JF, Ariyawansa HA, Baghela A, Bahkali AH, Beug M, Bhat DJ, Bojantchev D, Boonpratuang T, Bulgakov TS, Camporesi E, Boro MC, Ceska O, Chakraborty D, Chen JJ, Chethana KWT, Chomnunti P, Consiglio G, Cui BK, Dai DQ, Dai YC, Daranagama DA, Das K, Dayarathne MC, De Crop E, De Oliveira RJV, de Souza CAF, de Souza JI, Dentinger BTM, Dissanayake AJ, Doilom M, Drechsler-Santos ER, Gho- bad-Nejhad M, Gilmore SP Gdoes-Neto A, Gorczak M, Haitjema CH, Hapuarachchi KK, Hashimoto A, He MQ, Henske JK, Hirayama K, Iribarren MJ, Jayasiri SC, Jayawardena RS, Jeon SJ, Jer6nimo GH, Jesus AL, Jones EBG, Kang JC, Karunarathna SC, Kirk PM, Konta S, Kuhnert E, Langer E, Lee HS, Lee HB, Li WJ, Li XH, Liimatainen K, Lima DX, Lin CG, Liu JK, Liu XZ, Liu ZY, Luangsa-ard JJ, Licking R, Lumbsch HT, Lumyong S, Leano EM, Marano AV, Matsumura M, McKenzie EHC, Mongkolsamrit S, Mortimer PE, Nguyen TTT, Niskanen T, Norphanphoun C, O'Malley MA, Parnmen S, Pawtowska J, Perera RH, Phookamsak R, Phukhamsakda C, Pires-Zottarelli CLA, Raspé O, Reck MA, Rocha SCO, de Santiago ALCMA, Senanayake IC, Setti L, Shang QJ, Singh SK, Sir EB, Solomon KV, Song J, Srikitikulchai P Stadler M, Suetrong S, Takahashi H, Takahashi T,
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 220
Kamran Habib et al.: A new Diatrypaceous fungal genus
Tanaka K, Tang LP Thambugala KM, Thanakitpipattana D, Theodorou MK, Thongbai B, Thummarukcharoen T, Tian Q, Tibpromma S, Verbeken A, Vizzini A, Viasak J, Voi- gt K, Wanasinghe DN, Wang Y, Weerakoon G, Wen HA, Wen TC, Wijayawardene NN, Wongkanoun S, Wrzosek M, Xiao YP, Xu JC, Yan JY, Yang J, Da Yang S, Hu Y, Zhang JF, Zhao J, Zhou LW, Persoh D, Phillips AJL, Maharachchikumbura SSN (2016) Fungal diversity notes 253-366: Taxonomic and phylogenetic contributions to fungal taxa. Fungal Diversity 78(1): 1-237. https://doi.org/10.1007/s13225-01 6-0366-9
Li XH, Wu HX, Li JC, Song JY, Wang Q, Promputtha |, Wijayawardene NN (2022) Two new species of Allodiatrype from monsoon evergreen broad-leaved forest in Pu’er, Yunnan, China. Warasan Khana Witthayasat Maha Witthayalai Chiang Mai 49(3): 565-580. https://doi.org/10.12982/CMJS.2022.047
Li QR, Long SH, Lin Y, Wijayawardene NN, Kang JC, Kumla J (2023) Diversity, morpholo- gy, and molecular phylogeny of Diatrypaceae from southern China. Frontiers in Micro- biology 14: 1140190. https://doi.org/10.3389/fmicb.2023.1140190
Liu JK, Hyde KD, Jones EG, Ariyawansa HA, Bhat DJ, Boonmee S, Maharachchikumbu- ra SS, McKenzie EH, Phookamsak R, Phukhamsakda C, Shenoy BD (2015) Fungal diversity notes 1-110: taxonomic and phylogenetic contributions to fungal species. Fungal Diversity 72: 1-97. https://doi.org/10.1007/s13225-01 5-0324-y
Liu W, Hui C, Wang F, Wang M, Liu G (2018) Review of the Resources and Utilization of Bamboo in China. Bamboo-current and future prospects, InTech, 133-142. https:// doi.org/10.5772/intechopen.76485
Long §S, Liu L, Pi Y, Wu Y, Lin Y, Zhang X, Long Q, Kang Y, Kang J, Wijayawardene NN, Wang F, Shen X, Li Q (2021) New contributions to Diatrypaceae from karst areas in China. Mycokeys 83: 1-37. https://doi.org/10.3897/mycokeys.83.68926
Luque J, Garcia-Figueres F, Legorburu FJ, Muruamendiaraz A, Armengol J, Trouillas FP (2012) Species of Diatrypaceae associated with grapevine trunk diseases in Eastern Spain. Phytopathologia Mediterranea 1: 528-540.
Lynch SC, Eskalen A, Zambino PJ, Mayorquin JS, Wang DH (2013) Identification and pathogenicity of Botryosphaeriaceae species associated with coast live oak (Quer- cus agrifolia) decline in southern California. Mycologia 105(1): 125-140. https://doi. org/10.3852/12-047
Ma H-X, Yang Z-E, Song Z-K, Qu Z, Li Y, Zhu AH (2023) Taxonomic and phylogenetic contributions to Diatrypaceae from southeastern Tibet in China. Frontiers in Microbi- ology 14: 1073548. https://doi.org/10.3389/fmicb.2023.1073548
Maharachchikumbura SSN, Wanasinghe DN, Elgorban AM, Al-Rejaie SS, Kazerooni EA, Cheewangkoon R (2022) Brunneosporopsis yunnanensis gen. et sp. nov. and Alloc- ryptovalsa xishuangbanica sp. nov., new terrestrial Sordariomycetes from Southwest China. Life (Basel, Switzerland) 12(5): 635. https://doi.org/10.3390/life1 2050635
Mehrabi M, Hemmati R, Vasilyeva LN, Trouillas FP (2015) A new species and a new re- cord of Diatrypaceae from Iran. Mycosphere: Journal of Fungal Biology 6(1): 60-68. https://doi.org/10.5943/mycosphere/6/1/7
Mehrabi M, Hemmati R, Vasilyeva LN, Trouillas FP (2016) Diatrypella macrospora sp. nov. and new records of diatrypaceous fungi from Iran. Phytotaxa 252(1): 43-55. https://doi.org/10.11646/phytotaxa.252.1.4
Mehrabi M, Bita A, Roghayeh H (2019) Two new species of Eutypella and a new combi- nation in the genus Peroneutypa (Diatrypaceae). Mycological Progress 18(8): 1057- 1069. https://doi.org/10.1007/s11557-019-01503-4
Miller MA, Pfeiffer W, Schwartz T (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. 2010 Gateway Computing Environments
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 271
Kamran Habib et al.: Anew Diatrypaceous fungal genus
Workshop (GCE), New Orleans, Louisiana, 14 Nov 2010. IEEE, New York, 1-8. https:// doi.org/10.1109/GCE.2010.5676129
Nylander JAA (2004) MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University, Uppsala.
O'Donnell K, Cigelnik E (1997) Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Molecular Phylo- genetics and Evolution 7(1): 103-116. https://doi.org/10.1006/mpev.1996.0376
Paolinelli-Alfonso M, Serrano Gomez C, Hernandez-Martinez R (2015) Occurrence of Eutypella microtheca in grapevine cankers in Mexico. Phytopathologia Mediterranea 54: 86-93.
Peng MK, Zhang B, Qu Z, Li Y, Ma HX (2021) New record genus and a new species of Allodiatrype from China based on morphological and molecular characters. Phyto- taxa 500(4): 275-284. https://doi.org/10.11646/phytotaxa.500.4.3
Persoh D, Melcher M, Graf K, Fournier J, Stadler M, Rmbold G (2009) Molecular and morphological evidence for the delimitation of Xylaria hypoxylon. Mycologia 101(2): 256-268. https://doi.org/10.3852/08-108
Phookamsak R, Hyde KD, Jeewon R, Bhat DJ, Jones EBG, Maharachchikumbura SSN, Raspé O, Karunarathna SC, Wanasinghe DN, Hongsanan S, Doilom M, Tennakoon DS, Machado AR, Firmino AL, Ghosh A, Karunarathna A, Mesié A, Dutta AK, Thongbai B, Devadatha B, Norphanphoun C, Senwanna C, Wei D, Pem D, Ackah FK, Wang G-N, Jiang H-B, Madrid H, Lee HB, Goonasekara ID, Manawasinghe IS, Kusan I, Cano J, Gené J, Li J, Das K, Acharya K, Raj KNA, Latha KPD, Chethana KWT, He M-Q, Duenhas M, Jadan M, Martin MP Samarakoon MC, Dayarathne MC, Raza M, Park MS, Telleria MT, Chaiwan N, Matocec N, de Silva NI, Pereira OL, Singh PN, Manimohan P, Uniyal P Shang Q-J, Bhatt RP Perera RH, Alvarenga RLM, Nogal-Prata S, Singh SK, Vadthanarat S, Oh S-Y, Huang S-K, Rana S, Konta S, Paloi S, Jayasiri SC, Jeon SJ, Mehmood T, Gibertoni TB, Nguyen TTT, Singh U, Thiyagaraja V, Sarma VV, Dong W, Yu X-D, Lu Y-Z, Lim YW, Chen Y, Tkaléec Z, Zhang Z-F, Luo Z-L, Daranagama DA, Thambugala KM, Tibpromma S, Camporesi E, Bulgakov TS, Dissanayake AJ, Senanayake IC, Dai DQ, Tang L-Z, Khan S, Zhang H, Promputtha I, Cai L, Chomnunti P Zhao R-L, Lumyong S, Boonmee S, Wen T-C, Mortimer PE, Xu J (2019) Fungal diversity notes 929-1035: Taxonomic and phylogenetic contributions on genera and species of fungi. Fungal Diversity 95(1): 1-273. https://doi.org/10.1007/s13225-019-00421-w
Phukhamsakda C, Nilsson RH, Bhunjun CS, de Farias ARG, Sun Y-R, Wijesinghe SN, Raza M, Bao D-F, LuL, Tibpromma S, Dong W, Tennakoon DS, Tian X-G, Xiong Y-R, Karunarath- na SC, Cai L, Luo Z-L, Wang Y, Manawasinghe IS, Camporesi E, Kirk PM, Promputtha I, Kuo C-H, Su H-Y, Doilom M, Li Y, Fu Y-B Hyde KD (2022) The numbers of fungi: Contri- butions from traditional taxonomic studies and challenges of metabarcoding. Fungal Diversity 114(1): 327-386. https://doi.org/10.1007/s13225-022-00502-3
Rambaut A (2012) FigTree: Tree Figure drawing tool 2006-2012, version 1.4.0. Inst Evol Biol, Univ Edinburgh, Edinburgh.
Rappaz F (1987) Taxonomie et nomenclature des Diatrypacees a asques octospores. Mycologia Helvetica 2: 285-648.
Rehm H (1907) Ascomycetes novi. Annales Mycologici 5: 516-546.
Rolshausen PE, Mahoney NE, Molyneux RJ, Gubler WD (2006) A reassessment of the species concept in Eutypa /ata, the causal agent of Eutypa dieback of grapevine. Phy- topathology 96(4): 369-377. https://doi.org/10.1094/PHYTO-96-0369
Ronquist F, Teslenko M, Van Der Mark P, Ayres DL, Darling A, Hohna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: Efficient Bayesian phylogenetic
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 999
Kamran Habib et al.: A new Diatrypaceous fungal genus
inference and model choice across a large model space. Systematic Biology 61(3): 539-542. https://doi.org/10.1093/sysbio/sys029
Samarakoon MC, Hyde KD, Maharachchikumbura SS, Stadler M, Gareth Jones EB, Promputtha |, Suwannarach N, Camporesi E, Bulgakov TS, Liu JK (2022) Taxonomy, phylogeny, molecular dating and ancestral state reconstruction of Xylariomycetidae (Sordariomycetes). Fungal Diversity 112(1): 1-88. https://doi.org/10.1007/s13225- 021-00495-5
Senanayake IC, Maharachchikumbura SS, Hyde KD, Bhat JD, Jones EG, McKenzie EH, Dai DQ, Daranagama DA, Dayarathne MC, Goonasekara ID, Konta S, Li WJ, Shang QJ, Stadler M, Wijayawardene NN, Xiao YP Norphanphoun C, Li Q, Liu XZ, Bahkali AH, Kang JC, Wang Y, Wen TC, Wendt L, Xu JC, Camporesi E (2015) Towards unraveling relationships in Xylariomycetidae (Sordariomycetes). Fungal Diversity 73(1): 73-144. https://doi.org/10.1007/s13225-015-0340-y
Senanayake IC, Rathnayaka AR, Marasinghe DS, Calabon MS, Gentekaki E, Lee HB, Hurdeal VG, Pem D, Dissanayake LS, Wijesinghe SN, Bundhun D (2020) Morphologi- cal approaches in studying fungi: Collection; examination; isolation; sporulation and preservation. Mycosphere: Journal of Fungal Biology 11(1): 2678-2754. https://doi. org/10.5943/mycosphere/11/1/20
Senwanna C, Phookamsak R, Doilom M, Hyde KD, Cheewangkoon R (2017) Novel taxa of Diatrypaceae from Para rubber (Hevea brasiliensis) in northern Thailand; introducing a novel genus Allocryptovalsa. Mycosphere: Journal of Fungal Biology 8(10): 1835- 1855. https://doi.org/10.5943/mycosphere/8/10/9
Shang QJ, Hyde KD, Phookamsak R, Doilom M, Bhat DJ, Maharachchikumbura SS, Prom- puttha | (2017) Diatrypella tectonae, and Peroneutypa mackenziei, spp. nov. (Diatry- paceae) from northern Thailand. Mycological Progress 16(4): 463-476. https://doi. org/10.1007/s11557-01 7-1294-0
Shang QJ, Hyde KD, Jeewon R, Khan S, Promputtha |, Phookamsak R (2018) Morpho- molecular characterization of Peroneutypa (Diatrypaceae, Xylariales) with two novel species from Thailand. Phytotaxa 356(1): 1-18. https://doi.org/10.11646/phyto- taxa.356.1.1
Stamatakis A (2014) RAxML Version 8: A tool for phylogenetic analysis and post-analy- sis of large phylogenies. Bioinformatics (Oxford, England) 30(9): 1312-1313. https:// doi.org/10.1093/bioinformatics/btu033
Thiyagaraja V, Senanayake IC, Wanasinghe DN, Karunarathna SC, Worthy FR, To-Anun C (2019) Phylogenetic and morphological appraisal of Diatrype lijiangensis sp. nov. (Diatrypaceae, Xylariales) from China. Asian Journal of Mycology 2(1): 198-208. https://doi.org/10.5943/ajom/2/1/10
Thambugala KM, Daranagama DA, Phillips AJ, Bulgakov TS, Bhat DJ, Camporesi E, Bah- kali AH, Eungwanichayapant PD, Liu ZY, Hyde KD (2017) Microfungi on Tamarix. Fun- gal Diversity 82(1): 239-306. https://doi.org/10.1007/s13225-016-0371-z
Trouillas FR Gubler WD (2004) Identification and characterization of Eutypa leptoplaca, a new pathogen of grapevine in Northern California. Mycological Research 108(10): 1195-1204. https://doi.org/10.1017/S0953756204000863
Trouillas FP. Gubler WD (2010) Host range, biological variation, and phylogenetic diver- sity of Eutypa lata in California. Phytopathology 100(10): 1048-1056. https://doi. org/10.1094/PHYTO-02-10-0040
Trouillas FR Urbez-Torres JR, Gubler WD (2010) Diversity of diatrypaceous fungi as- sociated with grapevine canker diseases in California. Mycologia 102(2): 319-336. https://doi.org/10.3852/08-185
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 223
Kamran Habib et al.: A new Diatrypaceous fungal genus
Trouillas FPR Pitt WM, Sosnowski MR, Huang R, Peduto F, Loschiavo A, Savocchia S, Scott ES, Gubler WD (2011) Taxonomy and DNA phylogeny of Diatrypaceae asso- ciated with Vitis vinifera and other woody plants in Australia. Fungal Diversity 49(1): 203-223. https://doi.org/10.1007/s13225-011-0094-0
Trouillas FR Hand FP, Inderbitzin P Gubler WD (2015) The genus Cryptosphaeria in the western United States: Taxonomy, multilocus phylogeny and a new species, C. multi- continentalis. Mycologia 107(6): 1304-1313. https://doi.org/10.3852/15-115
U’'ren JM, Miadlikowska J, Zimmerman NB, Lutzoni F, Stajich JE, Arnold AE (2016) Contributions of North American endophytes to the phylogeny, ecology, and taxonomy of Xylariaceae (Sordariomycetes, Ascomycota). Molecular Phylogenetics and Evolution 98: 210-232. https://doi.org/10.1016/j.ympev.2016.02.010
Urbez-Torres JR, Adams P Kamas J, Gubler WD (2009) Identification, incidence, and pathogenicity of fungal species associated with grapevine dieback in Texas. Amer- ican Journal of Enology and Viticulture 60(4): 497-507. https://doi.org/10.5344/ ajev.2009.60.4.497
Urbez-Torres JR, Peduto F, Striegler RK, Urrea-Romero KE, Rupe JC, Cartwright RD, Gubler WD (2012) Characterization of fungal pathogens associated with grapevine trunk diseases in Arkansas and Missouri. Fungal Diversity 52(1): 169-189. https:// doi.org/10.1007/s13225-011-0110-4
Vieira ML, Hughes AF, Gil VB, Vaz AB, Alves TM, Zani CL, Rosa CA, Rosa LH (2011) Diversity and antimicrobial activities of the fungal endophyte community associated with the traditional Brazilian medicinal plant Solanum cernuum Vell. (Solanaceae). Canadian Journal of Microbiology 58(1): 54—56. https://doi.org/10.1139/w11-105
Vu D, Groenewald M, De Vries M, Gehrmann T, Stielow B, Eberhardt U, Al-Hatmi A, Groenewald JZ, Cardinali G, Houbraken J, Boekhout T, Crous PW, Robert V, Verkley GJM (2019) Large-scale generation and analyses of filamentous fungal DNA bar- codes boosts coverage for kingdom fungi and reveals thresholds for fungal spe- cies and higher taxon delimitation. Studies in Mycology 92(1): 135-154. https://doi. org/10.1016/j.simyco.2018.05.001
White TJ, Burns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fun- gal ribosomal RNA genes for phylogenetics. PCR Protocols, a Guide to Methods and Applications, Academic, San Diego, 315-322. https://doi.org/10.1016/B978-0-12- 372180-8.50042-1
Wijayawardene NN, Hyde KD, Tibpromma S, Wanasinghe DN, Thambugala KM, Tian Q, Wang Y (2017) Towards incorporating asexual fungi in a natural classification: Check- list and notes 2012-2016. Mycosphere: Journal of Fungal Biology 8(9): 1457-1555. https://doi.org/10.5943/mycosphere/8/9/10
Wijayawardene NN, Hyde KD, Dai DQ, Sanchez-Garcia M, Goto BT, Saxena RK, Erdogdu M, Selcuk F, Rajeshkumar KC, Aptroot A, Btaszkowski J, Boonyuen N, da Silva GA, de Souza FA, Dong W, Ertz D, Haelewaters D, Jones EBG, Karunarathna SC, Kirk PM, Kukwa M, Kumla J, Leontyev DV, Lumbsch HT, Maharachchikumbura SSN, Marguno F, Martinez-Rodriguez P Mesié A, Monteiro JS, Oehl F, Pawtowska J, Pem D, Pfliegler WP, Phillips AJL, Posta A, He MQ, Li JX, Raza M, Sruthi OP Suetrong S, Suwannarach N, Tedersoo L, Thiyagaraja V, Tibpromma S, Tkalcec Z, Tokarev YS, Wanasinghe DN, Wijesundara DSA, Wimalaseana SDMK, Madrid H, Zhang GQ, Gao Y, Sanchez-Castro |, Tang LZ, Stadler M, Yurkov A, Thines M (2022) Outline of Fungi and fungus-like taxa — 2021. Mycosphere: Journal of Fungal Biology 13(1): 53-453. https://doi. org/10.5943/mycosphere/13/1/2
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 224
Kamran Habib et al.: A new Diatrypaceous fungal genus
Xie X, Liu LL, Shen XC, Kang YQ, Hyde KD, Kang JC, Li QR (2020) Contributions to spe- cies of Xylariales in China-3. Collodiscula tubulosa (Xylariaceae). Phytotaxa 428(2): 122-130. https://doi.org/10.11646/phytotaxa.428.2.6
Yang Z, Zhang B, Qu Z, Song Z, Pan X, Zhao C, Ma H (2022) Two new species of Diatrype (Xylariales, Ascomycota) with polysporous asci from China. Diversity 14(2): 149. https://doi.org/10.3390/d14020149
Zhu H, Pan M, Wijayawardene NN, Jiang N, Ma R, Dai D, Tian C, Fan X (2021) The hid- den diversity of Diatrypaceous fungi in China. Frontiers in Microbiology 12: 646262. https://doi.org/10.3389/fmicb.2021.646262
Supplementary material 1
Alignment file
Authors: Kamran Habib, Xin Zhou, Wenyu Zeng, Xu Zhang, Hongmin Hu, Qianzhen Wu, Lili Liu, Yan Lin, Xiangchun Shen, Jichuan Kang, Qirui Li
Data type: fasta
Copyright notice: This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.
Link: https://doi.org/10.3897/mycokeys.108.126712.suppl1
MycoKeys 108: 197-225 (2024), DOI: 10.3897/mycokeys.108.126712 995